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9 octobre 2020: 1 événement

  • Soutenances de Thèse/HDR

    Vendredi 9 octobre 2020 10:00-13:00 -

    PhD - Dwiria Wahyuni

    Résumé : Quantitative analysis in microscopy imaging has always been a challenge. One noticeable quantitative technique is Fluorescence Fluctuation Microscopy, a family of analytical tools generally developed for confocal microscopes that takes advantage of the temporal and/or spatial fluctuations of the fluorescence signal emitted by molecules, Firstly, we developed an approach that combines Image Correlation Spectroscopy with photobleaching to better estimate the surface density of immobilized molecules. The method is useful to overcome the limitation of the standard Image Correlation Spectroscopy when applied to systems of molecules with multi-labeling or aggregates. It has been successfully tested on fluorescence beads that exhibit a wide distribution of brightness, then applied to proteins of the extracellular matrix deposited on the substrate and oligomerization of protein in cell cytoplasm. Secondly, we performed Raster Image Correlation Spectroscopy on CRY2/CIBN optogenetics cells. Since the technique covers a wide range au diffusional time scales, it is useful to measure the diffusion constant of the cytoplasmic CRY2 proteins and membranous CIBN proteins. We also managed to characterize the dissociation process of CRY2/CIBN.

    En savoir plus : Soutenances de Thèse/HDR