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Accueil > Équipes > OPTique et IMAgeries > Instrumentation et Méthodes > Imaging systems

Super-resolution photoacoustic microscopy

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Improving the performance of the Optical Resolution Photoacoustic Microscopy (ORPAM) is an interesting goal since presently it is not as good as in fluorescence microscopy. We will focus on the first instant of the photoacoustic signal generation : from the excitation of the upper states of chromophores to the non radiative relaxation and phonon emission. Excited State Absorption (ESA) and its more powerful variant the Reverse Saturable Aborption (RSA) is able to provide a quadratic power dependence equivalent, but more efficient than to the non resonant two-Photon excitation process, leading to an optical sectioning which is missing in ORPAM. Stimulated Emission Depletion (STED) is a way to reach the super-resolution in fluorescence microscopy. We will introduce the STED effect in ORPAM by frustating the phonon emission via the depletion of the excited state population.