A major focus of this project consists in investigating the influence of several parameters (cell shape, intercellular junction size, energy of stimulation) that might impact the response of a cell to its neighbor contractility.
Our approach involves the use of micropatterns on soft hydrogels for guiding cell adhesion and shape. Using this technique, we are able to measure both fine-scale cytoskeletal organization and cell-generated forces. The contractility of one cell will be modulated by optogenetics, i.e. light stimulation of a protein regulating cell contractility. The micropatterns will be fabricated by photo-patterning of polyacrylamide gels. The cell-generated forces will be assessed by traction force microscopy. The characterization of the cell response to stimulation will be done by using a digital micro- mirror device (DMD) to control the shape of the stimulating light.
Location :
The candidate will work at the Laboratory for Interdisciplinary Physics (LIPhy), in the MOTIV team (Materials, Optics and Instrumental Techniques for the Life Sciences).
LIPhy : https://www-liphy.ujf-grenoble.fr/
Profile & requested skills :
5th year engineering school student and / or Master student whose training focuses primarily on biophysics, tissue engineering, biology and biomaterials.
We are looking for highly motivated scientists with an aptitude for teamwork and good communication skills in oral and written English.
Subject could be continued with a PhD thesis.
Allowance :
Internship allowance will be provided ( 500€/month)
CONTACT
Please send a CV + a cover letter (including names/contact email of 2 referees) + the record of your grades of the 2 past academic years (2015/2016 & 2016/2017) to
Martial Balland (martial.balland@univ-grenoble-alpes.fr) and
Thomas Boudou (thomas.boudou@univ-grenoble-alpes.fr).
