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Mechanical properties of spheroids using AFM

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Spheroids are good models to describe the behaviour of solid tumours located within the human body. Although they have been studied extensively, it is not clear yet how their rheological properties change with growth (or size), how they depend on the presence of extra-cellular matrix, and how cell invasiveness can come into play.

Here we propose to study the viscoelasticity of such speroids (made of invasive cancer cells of different grades) in the presence of extracellular matrix (ECM), using a home-made technique based on the use of the AFM [1,2]. Confocal microscopy will also be used to monitor the structure of spheroids and the location of the ECM. Further developments will be made with collaborators to propose a proper rheological model [3].

Contact :

Claude Verdier : claude.verdier@univ-grenoble-alpes.fr, LIPHY, Grenoble

References :

[1] Y. Abidine, A. Constantinescu, V.M. Laurent, V. Sundar Rajan, R. Michel, V. Laplaud, A. Duperray, C. Verdier, Mechanosensitivity of cancer cells in contact with soft substrates using AFM, Biophys. J., 114, 1165-1175 (2018)
[2] A. Giannetti, J. Revilloud, C. Verdier, Mechanical properties of 3D tumor spheroids measured by AFM, to appear in Comput. Methods Biomechanics Biomed. Eng (2020)
[3] M. Dolega, G. Zurlo, B. Brunet, M. Le Goff, M. Greda, C. Verdier, J.-F. Joanny, G. Cappello, P. Recho, Mechanical behavior of multi-cellular spheroids under osmotic compression, Submitted (2020)

Site web :

https://www-liphy.univ-grenoble-alpes.fr/pagesperso/verdier/