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Accueil > Équipes > Fluctuations, Régulations et Evolution des systèmes vivants > Thèmes de l’équipe

Gene regulation by the RpoS sigma factor of E. coli

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We study the global regulatory effects of RpoS and its cofactors (Crl and an uncharacterized protein) in E. coli K12. In particular, we are looking for antisens and non-coding RNAs (RNAseq) and transcription factors responsible of indirect gene regulation, especially negative regulation. We are also trying to identify the core RpoS regulon (Run Off Micro Array, ChIP-seq) in order to determine specific DNA features and properties of strictly RpoS-dependent promoters.


Stephan Lacour


Paolo Landini, Università degli Studi di Milano, Italy
Clelia Peano, ITB, National Research Council, Segrate (MI), Italy
Magne Osteris, Fasteris, Geneva, Switzerland

Related Publications

  • Peano C., Wolf J., Demol J., Rossi E., Petiti L., De Bellis G., Geiselmann J., Egli T., Lacour S, Landini P., Characterization of the Escherichia coli sigmaS core regulon by Chromatin Immunoprecipitation-sequencing (ChIP-seq) analysis. Sci. Rep. 2015 ; 5 : 10469 ; DOI : 10.1038/srep10469. [link]
  • Landini P., Egli T., Wolf J., Lacour S., SigmaS, a major player in the response to environmental stresses in Escherichia coli : role, regulation and mechanisms of promoter recognition. Envir. Microbiol. Rep. 2014 ; DOI : 10.1111/1758-2229.12112. [link]
  • Dudin O., Lacour S., Geiselmann J., Expression dynamics of RpoS/Crl-dependent genes in Escherichia coli. Res. in Microbiol. 2013 ; DOI : 10.1016/j.resmic.2013.07.002. [link]
  • Lacour S., Leroy, O., Kolb A., Landini P., Substitutions in region 2.4 of σ70 allow recognition of the σS-dependant aidB promoter. J. Biol. Chem. 2004 ; Dec 31 ; 279(53):55255-61. [link]
  • Lacour S., Landini P., σS-dependent gene expression at the onset of stationary phase in Escherichia coli : function of σS-dependent genes and identification of their promoter sequences. J. Bacteriol. 2004 ; Nov ; 186(21):7186-95. [link]
  • Lacour S., Kolb A., Landini P., Nucleotides from –16 to –12 determine specific promoter recognition by bacterial σS-RNA polymerase. J. Biol. Chem. 2003 ; Sep 26 ; 278(39):37160-8. [link]
  • Lacour S., Zehnder A.J.B., Kolb A., Landini P., Mechanism of specific recognition of the aidB promoter by EσS-RNA polymerase. Biochem. Biophys. Res .Commun. 2002 ; Apr 12 ; 292(4):922-30. [link]