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Accueil > Équipes > OPTique et IMAgeries > Physics and imaging for life science > Cell dynamics and signaling

Fluorescence fluctuation microscopy

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Cell response at the molecular scale can also be probed using fluorescence fluctuations microscopy (FFM), a family of methods derived from fluorescence correlation spectroscopy (FCS). These methods exploit the statistical properties of detected fluorescence, in single points or images, to obtain information about molecular mobility and aggregation state. In collaboration with biologists (O. Destaing, IAB, Grenoble), the group works on optogenetic systems (molecular interactions that are activated by light) : for example, optogenetics can be used to activate different cell response by modulating the oligomerization state of signaling molecules that can be quantified by FFM. Fluctuation-based techniques also led to the emergence of a technology-transfer project, ConfoBright, coordinated by A. Delon. It aims at supporting innovation for cancer research at IAB. This unique instrument combines a confocal microscope, a customized AO system and a 2-photon laser (Fig. 2, B). We will develop multi-metric strategies, where spatial properties such as contrast or intensity (i.e. image metrics) are combined with local measurements (i.e. fluctuation metrics), that can be tailored to answer specific biological questions. Various projects at IAB will benefit from the ConfoBright system : profound imaging in cell spheroids, 3D assessment of macrophages phagocytosis of cancerous cells, chromatin organization, nuclear shape and cancer, ultra-fast 3D confocal imaging of rapid calcium events.