Laboratoire Interdisciplinaire de Physique

Optical excitation at the microscopic scale is used in a large variety of domains such as: the generation of the fluorescence of biological markers or the photochemistry in resins for the (...)

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Using light sheet microscopy(SPIM) we plan to shown that the STED effect using our DPSS-lasers will drastically improve the axial resolution. Indeed, as shown on the schematics of the LSFM (...)

As a first step our aim is not to perform imaging, the excitation-depletion processes are induced in a uniform distribution of fluorescence emitters, i.e. a Coumarin 490 dye solution and the (...)

Our first setup is based on a DPSS self-Q-switched Nd:YAG laser from TEEM-Photonics delivering 7 μJ nanosecond pulses at a rate of 20 kHz, we also investigate a more powerful model (100µJ) but (...)

Background & general objectives Microchip based microlaser is a perfect choice for the conception of a STED microscopy system. Its sub nanosecond pulse duration is well adapted to the (...)

A number of blue fluorophores have spectral properties matched to the wavelengths delivered by the bi-color laser(355 nm for excitation and 532 nm for stimulation) such as Coumarin 490 shown on (...)

For simple STED scanning microscopy we kept the two wavelengths merged along the whole optical path to preserve their intrinsic alignment. However, the STED beam is to display a dark center, (...)

The classic mask producing a quasi donut shape for the stimulating wavelength is mandatory for confocal-STED microscopy. For STED-SPIM, the stimulating light sheet can be obtained in a different and simpler way by using a mask that produces directly a zero intensity light sheet instead of a zero intensity central spot.